Save
Different pathophysiological relevance of branched fatty acid metabolism in mice and men: alpha-methylacyl-CoA racemase regulates the T cell response only in experimental autoimmune encephalomyelitis but not in multiple sclerosis
Author(s): ,
C.A. Mayer
Affiliations:
Neurology, Goethe University Frankfurt
,
N. Tafferner
Affiliations:
Fraunhofer Institute for Molecular Biology and Applied Ecology IME
,
J. Barthelmes
Affiliations:
Pharmazentrum Frankfurt/ZAFES, Goethe University Frankfurt, Frankfurt am Main, Germany
,
M. Eberle
Affiliations:
Pharmazentrum Frankfurt/ZAFES, Goethe University Frankfurt, Frankfurt am Main, Germany
,
N. Ferreiros
Affiliations:
Pharmazentrum Frankfurt/ZAFES, Goethe University Frankfurt, Frankfurt am Main, Germany
,
T. Ulshöfer
Affiliations:
Fraunhofer Institute for Molecular Biology and Applied Ecology IME
,
M. Henke
Affiliations:
Fraunhofer Institute for Molecular Biology and Applied Ecology IME
,
N. de Bruin
Affiliations:
Fraunhofer Institute for Molecular Biology and Applied Ecology IME
,
G. Geisslinger
Affiliations:
Fraunhofer Institute for Molecular Biology and Applied Ecology IME; Pharmazentrum Frankfurt/ZAFES, Goethe University Frankfurt, Frankfurt am Main, Germany
,
C. Foerch
Affiliations:
Neurology, Goethe University Frankfurt
,
M.J. Parnham
Affiliations:
Fraunhofer Institute for Molecular Biology and Applied Ecology IME; Pharmazentrum Frankfurt/ZAFES, Goethe University Frankfurt, Frankfurt am Main, Germany
S. Schiffmann
Affiliations:
Fraunhofer Institute for Molecular Biology and Applied Ecology IME; Pharmazentrum Frankfurt/ZAFES, Goethe University Frankfurt, Frankfurt am Main, Germany
ECTRIMS Online Library. Mayer C. Oct 8, 2015; 115431
Christoph Mayer
Christoph Mayer
Login now to access Regular content available to all registered users.

You may also access this content "anytime, anywhere" with the Free MULTILEARNING App for iOS and Android
Abstract
Discussion Forum (0)
Rate & Comment (0)
Abstract: P371

Type: Poster

Abstract Category: Immunology

Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS), therefore targeting the metabolism of immune cells is an attractive strategy to modify their function during autoimmunity in MS. We investigated the effects of modulating fatty acid metabolism in an animal model of multiple sclerosis, the experimental autoimmune encephalomyelitis (EAE). Alpha-methylacyl-CoA racemase (AMACR) prepares R-configurated branched fatty acids for metabolic ß-oxidation by coverting them into the S-configuration. We observed significant, disease-dependent elevation of AMACR expression in various immune cells from blood, draining lymph nodes and spleen in EAE mice during the preclinical phase. In vitro studies further revealed that genetic deletion of AMACR inhibits the proliferation of T cells. Activated T cells isolated from AMACR knockout (KO) mice are characterized by a higher production of IFN-γ, IL-17 and IL-10 and a lower production of IL-4 in comparison to T cells of wild type mice. AMACR-deficient mice showed a significant but, however, only slight worsening of early clinical symptoms of EAE in comparison to wild type mice. AMACR was not regulated in white blood cells of MS patients. Our data thus suggest that AMACR is regulated in immune cells during EAE but is neither essential for the development of EAE nor a relevant player in pathology of Multiple Sclerosis

Disclosure:

Christoph Mayer: Received travel grants from genzyme, biogen and Merck serono and speakers honoraria from genzyme, Merck serono and biogen

Nadja Tafferner: Nothing to disclose

Julia Barthelmes: Nothing to disclose

Max Eberle: Nothing to disclose

Nerea Ferreiros: Nothing to disclose

Thomas Ulshöfer: Nothing to disclose

Marina Henke: Nothing to disclose

Natasja deBruin: Nothing to disclose

Marina Henke: Nothing to disclose

Natasja deBruin: Nothing to disclose

Christian Foerch received travel grants from TEVA pharma, biogen and genzyme and speakers honoraria from genzyme and biogen

Gerd Geisslinger: Nothing to disclose

Michael J. Parnham: Nothing to disclose

Susanne Schiffmann: Nothing to disclose

Code of conduct/disclaimer available in General Terms & Conditions
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.


Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.



Google Analytics is used for user behavior tracking/reporting. Google Analytics works in parallel and independently from MLG’s features. Google Analytics relies on cookies and these cookies can be used by Google to track users across different platforms/services.


Save Settings