High intensity training may reverse the fiber type specific decline in myogenic stem cells in multiple sclerosis patients
Author(s): ,
I. Wens
Hasselt University, Diepenbeek, Belgium
J. Farup
Aarhus University, Aarhus, Denmark
C. Keytsman
Hasselt University, Diepenbeek, Belgium
B.O. Eijnde
Hasselt University, Diepenbeek, Belgium
U. Dalgas
Aarhus University, Aarhus, Denmark
ECTRIMS Online Library. Dalgas U. Oct 9, 2015; 116108; 1719
Ulrik Dalgas
Ulrik Dalgas
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Abstract: P813

Type: Poster

Abstract Category: MS symptoms

Background: Multiple sclerosis (MS) is associated with loss of skeletal muscle mass and function. The myogenic stem cells (satellite cells - SCs) are instrumental for accretion of additional nuclei to the post-mitotic muscle cells, but remains to be investigated in MS.

Objectives: The present study compared the SC and myonuclei content between MS patients (n=23) and age matched healthy controls (HC, n=18). Furthermore, the effects of 12 weeks of high intensity training on SC and myonuclei content were explored in MS.

Methods: Muscle biopsies were obtained from m. Vastus Lateralis at baseline (MS+HC) and following 12 weeks of training (MS only). Frozen biopsies were sectioned followed by immunohistochemical analysis for fiber type specific SCs (Pax7+), myonuclei (MN) and central nuclei content and fiber cross-sectional area (fCSA) using ATPase histochemistry.

Results: At baseline the SCs/fiber was lower in type II compared to type I fiber in both MS (119%, p< 0.01) and HC (69%, p< 0.05), whereas the SCs/fCSA was lower in type II fibers compared to type I only in MS (72%, p< 0.05). No differences were observed in MN or central nuclei between MS and HC. Following training the type II fiber SCs per fiber and fCSA in MS patients increased by 165% (p< 0.05) and 135% (p< 0.05), respectively. Furthermore, the type II fiber MN content increased by 35% (p< 0.05) following training.

Conclusions: In conclusion, the SC content is lower in type II compared to type I fibers in both MS and HC. Furthermore, high intensity training was observed to selectively increase the SC and myonuclei content in type II fibers in MS patients.


Inez Wens: nothing to disclose

Jean Farup: nothing to discclose

Charly Keytsman: nothing to disclose

Bert O. Eijnde: nothing to disclose

Ulrik Dalgas has received research support, travel grants and/or teaching honorary from Biogen Idec, Merck Serono and Sanofi Aventis.
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