Markers of oxidative stress – correlation with vitamin D3 and smoking in multiple sclerosis (MS) patients
Author(s): ,
T. Andjelic
Affiliations:
Institute of Medical Biochemistry
,
M. Mijuskovic
Affiliations:
Nephrology Clinic, Military Medical Academy
,
J. Kotur-Stevuljevic
Affiliations:
Institute of Medical Biochemistry, Faculty of Pharmacy
,
M. Milenkovic
Affiliations:
Beiersdorf
D. Obradovic
Affiliations:
Medical Faculty, Military Medical Academy, University of Defense, Belgrade, Serbia
ECTRIMS Online Library. Obradovic D. Oct 12, 2018; 228160; P1782
Dragana Obradovic
Dragana Obradovic
Contributions
Abstract

Abstract: P1782

Type: Poster Sessions

Abstract Category: N/A

Background: Cigarette smoking and vitamin D deficiency are well known risk factors for MS. Oxidative stress (OS) is implicated in pathophysiology of MS, and also elevated in smokers. The aim of our study was to investigate correlation between smoking, vitamin D3 and oxidative stress markers in MS patients during remission.
Methods: The study involved 111 relapse-remitting MS patients, 39 smokers (18 males and 21 females), aged 41.8 ± 7.8, EDSS 2.1 and 71 nonsmokers (37 males, 34 females), aged 42.7 ±9.4, EDSS 2.7. All patients were on vit D supplementation. We measured in plasma: advanced oxidation protein products (AOPP), prooxidative-antioxidative balance (PAB), malondialdehyde (MDA), nitrate and nitrite, superoxide dismutase (SOD), total antioxidative status (TAS), paraoxonase (PON) and transferrine. AOPP, PAB, MDA and nitrite and nitrate represent prooxidants, while SOD, TAS, PON and transferrine are antioxidant molecules. Serum levels of vitamin D3 were analyzed as 25(OH)vitamin D by chemiluminescence immunoassay.
Results: We found statistically significant differences between the smoking and non-smoking group in the levels of AOPP (26.7±7.9 vs 23.0±6.0 µmol/L), PAB (170.1±46.1 vs 139.3±44.2 HKU), MDA (10.0±2.9 vs 8.7±2.1 µmol/L), nitrate and nitrite (5.5±2.3 vs 4.3±1.8 µmol/L), TAS (897±217 vs 995±189 µmol/L), SOD (712±156 vs 857±319 U/L) , transferrine (2.5±0.4 vs 2.7±0.5 g/L) and vitamin D: 62.7(34.5-90.3) vs 98.7(47.2-118.4) nmol/L. There was no statistically significant difference in paraoxonase acitivity between smokers and nonsmokers: 378(183-568) vs 452(209-648) U/L.
Conclusion: Higher levels of prooxidants, lower levels of antioxidant markers and lower vitamin D3 levels were found in smokers as compared to nonsmokers. Cigarette smoking potentiates OS, decreases antioxidant protection and correlates with lower vitamin D3 levels in MS patients during remission. All of these might have an unfavorable impact on MS course
Disclosure: Tamara Andjelic: nothing to disclose
Mirjana Mijuskovic: nothing to disclose
Jelena Kotur-Stevuljevic: nothing to disclose
Milica Milenkovic: nothing to disclose
Dragana Obradovic: nothing to disclose

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