Save
Short chain fatty acids levels in plasma of multiple sclerosis patients: a correlation with the disability of the disease and the activation of the immune system
Author(s): ,
S. Perez Perez
Affiliations:
Instituto Investigación Sanitaria San Carlos (IdISSC). Grupo de Investigación de EM. Hospital Clínico San Carlos
,
M.I. Domínguez Mozo
Affiliations:
Instituto Investigación Sanitaria San Carlos (IdISSC). Grupo de Investigación de EM. Hospital Clínico San Carlos
,
S. Medina
Affiliations:
Servicio de Inmunología. Hospital Universitario Ramón y Cajal / Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)
,
N. Villarubia
Affiliations:
Servicio de Inmunología. Hospital Universitario Ramón y Cajal / Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)
,
A. Alonso Gómez
Affiliations:
Instituto Investigación Sanitaria San Carlos (IdISSC). Grupo de Investigación de EM. Hospital Clínico San Carlos
,
M.Á. García Martínez
Affiliations:
Instituto Investigación Sanitaria San Carlos (IdISSC). Grupo de Investigación de EM. Hospital Clínico San Carlos
,
L. Costa Frossard
Affiliations:
Servicio de Inmunología. Hospital Universitario Ramón y Cajal / Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)
,
J.C. Álvarez Cermeño
Affiliations:
Servicio de Inmunología. Hospital Universitario Ramón y Cajal / Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)
,
R. Arroyo González
Affiliations:
Department of Neurology, Hospital Universitario Quironsalud Madrid, Madrid, Spain
,
L.M. Villar Guimerans
Affiliations:
Servicio de Inmunología. Hospital Universitario Ramón y Cajal / Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)
R. Álvarez Lafuente
Affiliations:
Instituto Investigación Sanitaria San Carlos (IdISSC). Grupo de Investigación de EM. Hospital Clínico San Carlos
ECTRIMS Online Library. Perez Perez S.
Oct 12, 2018; 228928
Silvia Perez Perez
Silvia Perez Perez
Login now to access Regular content available to all registered users.

You may also access this content "anytime, anywhere" with the Free MULTILEARNING App for iOS and Android
Abstract
Discussion Forum (0)
Rate & Comment (0)

Abstract: P1087

Type: Poster Sessions

Abstract Category: Pathology and pathogenesis of MS - Environmental factors

Background: Gut microbiota has been related to multiple sclerosis (MS) etiopathogenesis. Short-chain fatty acids (SCFA) are compounds derived from microbial metabolism that have a role in gut-brain axis. The most abundant SCFA are butyrate, propionate and acetate.
Objectives: To analyse SCFA plasma levels of MS patients and healthy donors (HD), and a possible link between these levels and both clinical characteristics of the pathology and immune cells populations.
Methods: 97 MS patients and 55 HD were included in this study. Patients were divided into two groups according to their EDSS (Expanded Disability Status Score), 50 with EDSS≤1.5 and 47 with EDSS≥5.0. Plasma samples were collected in order to analyse SCFA levels by liquid chromatography-mass spectrometry, and peripheral blood cells were isolated for flow cytometry analysis of immune cells populations.
Results: Acetate plasma levels were significantly higher in patients than in HD (p=0.002). Patients with EDSS≥5.0 had significantly higher acetate levels than those with EDSS≤1.5 (p=0.02), and than HD (p=1.79e-4), existing a correlation between acetate levels and EDSS (r=0.406, p=4.09e-5). In MS patients, every SCFA were correlated (propionate-acetate: r=0.443, p=6.81e-6; butyrate-acetate: r=0.315, p=0.002; propionate-butyrate: r=0.715, p=3.82e-16). Relating to flow cytometry analysis, the main associations were that MS patients with high acetate levels showed decreased CD4+ naïve T cells (r=-0.550, p=0.001) and increased CD8+ IL-17+ cells (r=0.556 p=0.001). In HD we did not find these associations, but high levels of propionate involved decreased B cells (r=-0.773, p=4.43e-4) and increased monocytes (r=0.613, p=0.001).
Conclusions: Acetate plasma levels are higher in MS patients than in HD. There exists a direct correlation between acetate plasma levels and EDSS in MS patients. In addition, high acetate levels seems to activate immune system in MS patients, favouring a Th17 response. Further studies are required to elucidate the mechanisms through which they could take part in the disease.
Disclosure: S. Pérez-Pérez: nothing to disclose.
M.I. Domínguez-Mozo: nothing to disclose.
S. Medina: nothing to disclose.
N. Villarubia: nothing to disclose.
A. Alonso-Gómez: nothing to disclose
M.A. García-Martínez: nothing to disclose.
L. Costa-Frossard: reports grants, personal fees and non-financial support from Merck-Serono, Teva, Sanofi-Aventis, Genzyme, Novartis, Biogen Idec and Roche
J.C. Álvarez-Cermeño: reports grants, personal fees and non-financial support from Bayer Schering, Biogen Idec, Genzyme, Novartis, Merck-Serono, Roche, Sanofi-Aventis and Teva
R. Arroyo: received honoraria for speaking and participating as investigators in clinical trials and non-financial support from Merck-Serono, Teva, Sanofi-Aventis, Genzyme, Novartis, Biogen Idec, Roche and Bayer-Schering.
L.M. Villar: reports grants, personal fees and non-financial support from Merck-Serono, Teva, Sanofi-Aventis, Genzyme, Novartis, Biogen Idec and Roche
R. Álvarez-Lafuente: reports grants and personal fees from Merck-Serono, personal fees and non-financial support from Biogen IDEC, grants, personal fees and non-financial support from Novartis Pharmaceuticals S.A., grants and personal fees from Genzyme, non-financial support from TEVA Pharma, S.L., non-financial support from Roche.

Code of conduct/disclaimer available in General Terms & Conditions
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.


Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.



Google Analytics is used for user behavior tracking/reporting. Google Analytics works in parallel and independently from MLG’s features. Google Analytics relies on cookies and these cookies can be used by Google to track users across different platforms/services.


Save Settings